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CyFlow BrettCount

Fast and reliable Brettanomyces spoiler monitoring

Test Kit for the specific detection of living Brettanomyces bruxellensis cells in wine and pre-mature wine sample. The specifically programmed VIT (VIT = Vermicon Identification Technology) gene probes bind to target structures within the corresponding living yeast in the wine sample material and deliver maximum specificity and speed for the detection.

At the ageing stage, wines are prone to a well-known threat to quality: contamination by Brettanomyces bruxellensis yeast. Without early detection of Brettanomyces, the production of undesirable volatile compounds with unpleasant aromas can mask or affect a wine’s bouquet.

Assurance of quality in wine production – with CyFlow BrettCount

A new detection system now enables the fast and reliable quality control testing for Brettanomyces.

CyFlow BrettCount allows detection of these spoilers much faster and with less effort than with classical culture methods. In addition, it detects the viable but not cultivable (VBNC) Brettanomyces, which are not detected even after seven days of culture.

Because CyFlow BrettCount directly detects living yeast cells, as one would see under a microscope, the result is not affected by inhibiting factors that can lead to an underestimation in other techniques. It also avoids false-positive results that can occur when using molecular biological methods, such as qPCR, in which stable fragments of DNA from dead yeast are amplified as well.

  • Technology:                   Fluorescence in-situ hybridization (FISH)
  • Specificity:                      Brettanomyces Bruxellensis
  • Time to result:               <3 hours.
  • Packaging:                      50 tests per kit
  • Storage:                          2-8 °C
  • Shelf life:                        12 months
+ Features & Benefits

At the ageing stage, wines are prone to a well-known threat to quality: contamination by Brettanomyces bruxellensis yeast. Without early detection of Brettanomyces, the production of undesirable volatile compounds with unpleasant aromas can mask or affect a wine’s bouquet.

Assurance of quality in wine production – with CyFlow BrettCount

A new detection system now enables the fast and reliable quality control testing for Brettanomyces.

CyFlow BrettCount allows detection of these spoilers much faster and with less effort than with classical culture methods. In addition, it detects the viable but not cultivable (VBNC) Brettanomyces, which are not detected even after seven days of culture.

Because CyFlow BrettCount directly detects living yeast cells, as one would see under a microscope, the result is not affected by inhibiting factors that can lead to an underestimation in other techniques. It also avoids false-positive results that can occur when using molecular biological methods, such as qPCR, in which stable fragments of DNA from dead yeast are amplified as well.

+ Specifications
  • Technology:                   Fluorescence in-situ hybridization (FISH)
  • Specificity:                      Brettanomyces Bruxellensis
  • Time to result:               <3 hours.
  • Packaging:                      50 tests per kit
  • Storage:                          2-8 °C
  • Shelf life:                        12 months
+ Documents